An efficient protocol towards site-specifically clickable nanobodies in high yield: cytoplasmic expression in Escherichia coli combined with intein-mediated protein ligation

Tien Ta, DuyDuyTien TaSteen Redeker, ErikErikSteen RedekerBillen, BrechtBrechtBillenReekmans, GunterGunterReekmansSikulu, JosephineJosephineSikuluNoben, Jean-PaulJean-PaulNobenGuedens, WandaWandaGuedensAdriaensens, PeterPeterAdriaensens2021-10-222021-10-2220151741-0126https://imec-publications.be/handle/20.500.12860/25994An efficient protocol towards site-specifically clickable nanobodies in high yield: cytoplasmic expression in Escherichia coli combined with intein-mediated protein ligationJournal article10.1093/protein/gzv032http://peds.oxfordjournals.org/content/28/10/351.long