High-definition microelectrode arrays with scalable, integrated microfluidics in multi-well format for drug screening on cardiomyocytes in a heart‑on‑a-chip application

Abstract

Towards increased throughput and automated workflows for organs-on-a-chip, a novel high-definition electrophysiology multi-well plate is developed in the Moore4Medical project. It consists of an advanced CMOS microelectrode array (MEA) chip with 16 sampling areas, each featuring 1024 electrodes, and of a polymeric fluidic cartridge with 16 corresponding microchambers, each connected to an integrated pneumatic peristaltic pump. It can perform 16-well assays with single-cell-resolution electrical recording and integrated microfluidics. Building on an earlier prototype, we present recent developments showing a scalable integration route and excellent fluidic and electrical functionalities of the plate in an easy-to-use system.