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Browsing by Author "Billen, Brecht"

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    An efficient protocol towards site-specifically clickable nanobodies in high yield: cytoplasmic expression in Escherichia coli combined with intein-mediated protein ligation

    Tien Ta, Duy
    ;
    Steen Redeker, Erik
    ;
    Billen, Brecht
    ;
    Reekmans, Gunter  
    ;
    Sikulu, Josephine
    Journal article
    2015, Protein Engineering, Design and Selection, (28) 10, p.351-363
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    Cytoplasmic versus periplasmic exprression of site-specifically and bioorthogonally functionalized nanobodies using expressed protein ligation

    Billen, Brecht
    ;
    Viincke, Cécile
    ;
    Hansen, Rebekka
    ;
    Devoogdt, Nick
    ;
    Muyldermans, Serge
    Journal article
    2017, Protein Expression and Purification, 133, p.25-34
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    Hydrazin-based expressed protein ligation for site specific modification of maltose binding protein

    Billen, Brecht
    ;
    Vranken, Tom
    ;
    Reekmans, Gunter  
    ;
    Steen Redeker, Erik
    ;
    Adriaensens, Peter  
    Oral presentation
    2014, ChemCYS
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    In situ monitoring and optimization of CuAAC-mediated protein functionalization of biosurfaces

    Vranken, Tom
    ;
    Steen Redeker, Erik
    ;
    Miszta, Adam
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    Billen, Brecht
    ;
    Hermens, Wim
    ;
    de Laat, Bas
    Journal article
    2017, Sensors and Actuators B: Chemical, 238, p.992-1000
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    Maltose binding protein as a model for site-specific modification by hydrazine-based expressed protein ligation for advanced biomaterials

    Billen, Brecht
    ;
    Vranken, Tom
    ;
    Reekmans, Gunter  
    ;
    Steen Redeker, Erik
    ;
    Adriaensens, Peter  
    Oral presentation
    2014, Biomedica 2014
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    Phosphodiester hydrogels for cell scaffolding and drug relaese applications

    Dera, Rafael
    ;
    Diliën, Hanne
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    Billen, Brecht
    ;
    Gagliari, Mick
    ;
    Rahimi, Nastaran
    ;
    van den Akker, Nynke
    Journal article
    2019, Macromolecular Bioscience, (19) 7, p.1900090
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    Protein engineering for directed immobilization

    Steen Redeker, Erik
    ;
    Ta, Duy Tien
    ;
    Cortens, David
    ;
    Billen, Brecht
    ;
    Guedens, Wanda  
    Journal article
    2013, Bioconjugate Chemistry, 24, p.1761-1777
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    Site-specific protein functionalization by expressed protein ligation for advanced biomaterials

    Billen, Brecht
    PHD thesis
    2017-12

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